MOLECULAR ANALYSIS OF BACTERIA ISOLATED FROM THE SOIL FOR ITS POTENTIAL AGNOSTIC ACTIVITY

Abstract

The work elucidates the agnostic action of a bacterial soil isolate, procured from different parts from Coimbatore, Tamilnadu, India. The bacteria strains were obtained via routine serial dilution plate protocols. The best isolate was tested against 3 pathogens; Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus. The screening was performed via streaking, and the isolate L1An1 exhibited agnostic activity on K pneumoniae and S aureus. The maximal impact of 21mm inhibition zone was recorded. Molecular identi?cation and the resultant sequence were consigned for NCBI BLAST. The isolates L1An2 showed 99% resemblance with that of Enterobacter aerogenes. The sequence submitted to GenBank and MT192658.1 was procured the accession number.