BIOTECHNOLOGICALAPPLICATION OF TALAROMYCES RADICUS ASSOCIATED WITH CUCUMIS DIPSACEUS EHRENB. EX SPACH

Abstract

Endophytes associated with the plants are rich source of secondary metabolites which represent a potential source of novel natural products for medicine, agricultural and industrial uses, such as antibiotics, anticancer agents, biological control agents and other bioactive compounds. The aim of the present work was to investigate the biotechnological potential of various extracts of Talaromyces radicus an endophyte from Cucumis dipsaceus. Talaromyces radicus was identified using colony morphology and 18S rDNA sequencing technique. Well grown endophytic fungus on potato dextrose broth was extracted sequentially with various solvents and the extracts were subjected to study their biological properties. Accordingly, primary and secondary phytochemicals, enzymes, organic acids and antimicrobial activity were evaluated. Talaromyces radicus showed the potential to produce phytochemicals like Flavonoids, Alkaloids, Phenols, Saponins and Tannins, Organic acids and Extracellular enzymes like Amylase, Cellulase, Protease and Lipase. Methanolic extract exerts potent antimicrobial activity against test pathogens followed by ethylacetate