TRIPODAL SCHIFF BASE TRIS [4-(4-NITROPHENYL)-3-AZA-3-BUTENYL] AMINE NANOROD FOR SELECTIVE DETECTION OF URIC ACID

Abstract

In this study, a tripodal Schiff base compound 3 (TNAA = Tris[4-(4-nitrophenyl)-3-aza-3-butenyl] amine) was synthesized, and its structure was confirmed using various techniques such as Fourier transform infrared spectroscopy (FTIR), UV–Visible spectroscopy (UV–Vis), Nuclear Magnetic Resonance (1H and 13C) spectroscopy, Electron Ionization Mass Spectroscopy (EIMS), Field Emission Scanning Electron Microscopy (FE-SEM) analysis and Electrochemical impedance spectroscopy (EIS). Cyclic and differential pulse voltammetry (CV and DPV) techniques were used to investigate the electrochemical sensing behavior of uric acid (UA) on a modified electrode (TNAA/GCE). At a potential of 0.42 V, the modified electrode detects the UA. The linear ranges of UA under optimal circumstances were 0.01 mM−4.96 mM, with a sensitivity of 24.17 µA cm2 mM−1. The observed limits of detection (LOD) and limit of quantification (LOQ) were 0.05 µM and 0.18 µM respectively. Interestingly, TNAA/GCE forecasts a greater current response after 50 cycles at 20 mV s−1, which also improves repeatability, reproducibility, and selectivity. The observed results predict that TNAA/GCE is a simple, cost-effective approach for detecting UA with high electrochemical sensitivity, superior repeatability, and long-term stability. Hence, the proposed sensor was used to perform real-time analysis on a human urine sample. Finally, a theoretical investigation of molecular docking was performed, and the binding affinity of Arthrobacter globiformis (Ag) uricase docked with TNAA using the Autodock tool was −16.5 Kcal/mol.