STANDARDIZATION OF A PROTOCOL FOR MICROPROPAGATION OF EUPATORIUM GLANDULOSUM L. AN IMPORTANT MEDICINAL PLANT

Abstract

Eupatorium glandulosum of Asteraceae family is an important medicinal plant used to cure various diseases by tribal populace. In the present study, a protocol for mass propagation of Eupatorium glandulosum has been developed from nodal explants cultured in the MS basal medium supplemented with various concentrations and combinations of IAA and BA. The maximum rate of proliferation of shoots per explants (59.66 ± 1.76) was observed in the medium containing 0.5 mg/l of IAA and 3 mg/l of BA, but medium with 1 mg/l of IAA and 4 mg/l of BA showed the maximum shoot length. When the regenerated shoots were rooted in vitro on MS basal medium supplemented with various concentrations of IAA and IBA, medium with 4 mg/l of IBA induced more number of roots per shoot (32.67 ± 0.89) while maximum root length (6.1 ± 0.12 cm) was recorded in 3 mg/l of IBA. The plantlets, with well developed root systems, were successfully acclimatized and transferred to the pots containing a mixture of Soil, Sand and Coco peat (1:1:1) with 100% survival rate.